Friday, June 10, 2005


Ferid Murad,

Welcome and Opening

Session 1
Molecular Mechanism

Chair: Ferid Murad, Houston
Harald Schmidt, Melbourne

Michael Marletta, Berkeley

Molecular steps in sGC activation

14:40 John Garthwaite, London
New vistas on NO-cGMP signaling
15:05 Teizo Kitagawa, Okazaki Resonance raman investigation of effects of YC-1 and GTP on structure of CO-bound heme of soluble guanylate cyclase

Session 2
CNS & Applications

Chair: John Garthwaite, London
Franz Hofmann, München
15:30 Vicente Felipo, Valencia Role of cGMP in learning: alternations in pathological situations and therapeutic implications
Martin Hendrix, Wuppertal Selective inhibitors of cGMP phosphodiesterases as procognitive agents
16:20 - 16:50
16:50 Urs Albrecht, Fribourg
Role of cGMP dependent protein kinases in the circadian system
David Morton, Portland
Atypical soluble guanylate cyclases in Drosophila as neuronal oxygen sensors and their involvement in gustation
David Raizen,
A cGMP-dependent protein kinase plays a pivotal role in the control of behavioral quiescence in C. elegans

Poster Session
18:00 - 20:00

Evening Lecture

Chair: Günter Schultz, Berlin
20:00 Joe Beavo, Seattle Regulation of cyclic nucleotide hydrolysis by cGMP

Saturday, June 11, 2005

Session 3
Cell Biology

Chair: Joe Beavo, Seattle
Michael Marletta, Berkeley
9:00 Doris Koesling, Bochum NO/cGMP signaling
9:25 Werner Müller-Esterl,
Reactive oxygen species induce tyrosine phosphorylation of and Src kinase recruitment to NO-sensitive guanylyl cyclase
9:50 Annie Beuve, Newark
Does sGC need a chaperone?

Session 4
cGMP Targets

Chair: Ulrich Walter, Würzburg
Peter Brouckaert, Gent
Jens Schlossmann, München Function of IRAG for cGMP kinase signaling in smooth muscle and platelets
Thomas Lincoln, Alabama
PKG regulation of smooth muscle gene expression
Wolfgang Dostmann, Burlington Exploring vasomotor mechanisms using PKG specific inhibitors
11:00 - 11:30
11:30 Renate Pilz,
La Jolla

Regulation of PKG expression in vascular smooth muscle cells

Joachim Schultz, Tübingen
Bacterial GAF-domaines

Session 5

Chair: John Burnett, Rochester
Johannes-Peter Stasch, Wuppertal
12:20 Christiane Rothkegel, Wuppertal Beyond NO and heme: biochemical and pharmacological opportunities
Ursula Schindler, Frankfurt
Role of activators of ferric sGC in cardiovascular disease
13:10 Barbara

The nitroxyl anion (NO-): a novel regulator of sGC and vascular tone
Nina Grosser,
Aspirin activates HO-1 expression in endothelial cells - role of NO/cGMP

Poster Session

Sunday, June 12, 2005

Session 6
Cardio-vascular Applications

Chair: Renate Pilz, La Jolla
Vicente Felipo, Valencia
9:00 John Burnett, Rochester cGMP enhancing strategies for acute and chronic heart failure
9:25 Michael Mendelsohn, Boston
Control of vascular remodeling by cGMP kinase
Ulrich Walter, Würzburg
Functional role of cGMP-dependent VASP phosphorylation in vascular cells
10:15 David Tulis,
NO-independent regulation of the vascular injury response
10:30 David Kass, Baltimore Role of PDE5a in cardiac stress response
10:55 - 11:25
John Parker, Toronto Differential effects of nitric oxide donors and phosphodiesterase V inhibitors on vascular function in humans

Session 7
Pulmonary Applications

Chair: Christian Schudt, Konstanz
Warren Zapol, Boston
11:50 Oleg Evgenov, Boston
sGC stimulator reverses acute pulmonary hypertension and augments the response to inhaled NO in awake lambs
12:15 Ardeschir Ghofrani, Giessen
sGC activation in pulmonary hypertension
Steve Abman, Denver
NO/cGMP signaling in the pathogenesis and treatment of persistent pulmonary hypertension in the newborn
13:05 - 14:00

Session 8
Renal Applications

Chair: Suzanne Lohmann, Würzburg
Adrian Hobbs, London
14:00 Michaela Kuhn, Würzburg
Endothelium-mediated actions of the ANP/cGMP system
Harm Peters,
ole of sGC in progressive renal fibrosis
Robert Carey, Charlottesville
Role of cGMP in natriuresis and pressure-natriuresis
Keith Thornbury,
Effects of stimulating the cGMP pathway on spontaneous activity in pacemaker cells isolated from the rabbit urethra
Wolf-Georg Forssmann, Hannover
Urodilatin, the renal natriuretic peptide: preclinical and clinical review

Session 9
Genetic Models

Chair: Michaela Kuhn, Würzburg
Werner Müller-Esterl, Frankfurt
Peter Brouckaert, Gent
Phenotypes of sGC deficient mice
16:00 - 16:30
Robert Feil, München Genetic dissection of signaling via cGMP-dependent protein kinases
Ferid Murad, Houston
Role of NO and cGMP in proliferation and differentiation of murine and human embryonic stem cells
Peter Ruth, Tübingen
Analysis of BKCa deficient mice

COCKTAILS AND DINNER Crown Estate of Bornstedt

Poster Session
  1. Abbey, Sarah E., Dmitri Smirnov, Lincoln R. Potter
    Differential regulation of NPR-B/GC-B by protein kinase C and calcium
  2. Antl, Melanie, Katja Sigl, Christina Eigelsperger, Matthias Werner, Franz Hofmann, Jens Schlossmann
    IRAG is involved in NO/cGMP-mediated inhibition of platelet function
  3. Antos, Laura K., Sarah E. Abbey-Hosch, Darcy R. Flora, Lincoln R. Potter
    ATP-independent activation of natriuretic peptide receptors
  4. Blume, Constanze, Kai Schuh, Peter Benz, Ulrich Walter, Thomas Renné
    In vivo modulation of vasodilator stimulated phosphoprotein functions by phosphorylation
  5. Boerrigter, Guido, Lisa C. Costello-Boerrigter, Harald Lapp, Johannes-Peter Stasch, John C. Burnett,
    Co-activation of soluble and particulate guanylate cyclase by BAY 58-2667 and BNP enhances cardiorenal function in experimental heart failure
  6. Bonartsev, Anton, Alexander Postnikov, Marina Artemieva, Natalia Medvedeva
    3-(3-[1,2,4]triazolo)-oxatriazolium-5-olate causes vasodilatation by NO-independent activation of soluble guanylate cyclase
  7. Buys, Emmanuel, Fumito Ichinose, John G. Morgan, Marielle Scherrer-Crosbie, Kenneth D. Bloch
    Myocyte-specific overexpression of NOS3 prevents endotoxin-induced myocardial dysfunction in mice
  8. Cawley, Sharon M. and Wolfgang R. Dostmann Kinetics of NO-induced Cyclic GMP responses in vascular smooth muscle
  9. Castro, Liliana, Grégoire Vandecasteele, Rodolphe Fischmeister
    PDE2 and PDE5 exert specific and distinct roles in regulating cGMP signals elicited by particulate or soluble guanylyl cyclases in rat cardiac myocytes
  10. Craven, Michael, Keith Thornbury, Gerard Sergeant, Mark Hollywood, Noel McHale
    Regulation of spontaneous activity in rabbit corpus cavernosum myocytes by nitric oxide.
  11. Day, Jonathan P., Miles D. Houslay, Shireen-A. Davies
    A novel role for cGMP-specific phosphodiesterase, PDE6, in active transport of cGMP
  12. De Vente, Jan, Marjanne Markerink-van Ittersum, James I. Gillespie
    NO-cGMP signaling in the guina pig bladder
  13. Deile, Martin, César Ibarra-Alvardo, Pavel I. Nedvetsky, Helmut Müller, Barbara Kemp-Harper, Johannes-Peter Stasch, H.H.H.W. Schmidt Effects of nitric oxide on sGC redox states
  14. Dhaese, Ingeborg, Gwen Vanneste, Patrick Sips, Emmanuel Buys, Peter Brouckaert, Romain Lefebvre
    NO-induced motility effects in distal colon of sGC a 1 knockout mice
  15. Domek-Lopacinska, Katarzyna, Marjanne Markerink-van Ittersum, Harry Steinbusch, Jan de Vente
    Changes in expression of cGMP selective phosphordiesterses 2, 5 and 9 in the rat brain during aging
  16. Dumitrascu, Rio, Norbert Weissmann, Hossein Ardeschir Ghofrani, Knut Beuerlein, Harald Schmidt, Johannes-Peter Stasch, Mark Jean Gnoth, Werner Seeger, Friedrich Grimminger, Ralph Theo Schermuly
    Activation of soluble guanylate cyclase reverses pulmonary vascular remodeling induced by hypoxia in mice
  17. Feil, Susanne, Patricia Zimmermann, Anna-Maria Knorn, Sabine Brummer, Jens Schlossmann, Franz Hofmann, Robert Feil
    Immunohistochemical analysis of cGKI expression in the murine brain and eye
  18. Friebe, Andreas, Evanthia Mergia, Oliver Dangel, Alexander Lange, Doris Koesling
    Gene inactivation of the β 1 subunit of NO-sensitive guanylyl cyclase in mice
  19. Gambaryan, Stepan, Michaela Kuhn, Ulrich Walter
    Inhibition of agonist-stimulated aldosterone production from adrenal zona glomerulosa cells by ANP is mediated by GC-A
  20. Garner-Hamrick, Peggy A., Karl Mathis, Tom Emmons, Michael A Tones
    Binding of [ 3H]BAY 58-2667 to purified bovine lung soluble guanylate cyclase by scintillation proximity assay
  21. González, Marta, Gonzalo Costa, Ángeles García-Pascual, Domingo Triguero
    Cyclic GMP-gated channels are co-localized with a population of Interstitial cells, but not with neuronal NOS, in the rat urethra
  22. Hallén, Katarina, Lars E. Gustafsson, N. Peter Wiklund
    Nerve­induced release of nitric oxide from the rabbit corpus cavernosum is modulated by cyclic GMP
  23. Hepp, Régine, Nicolas Gervasi, Ludovic Tricoire, Bertrand Lambolez, Danièle Tritsch, Pierre Vincent
    Imaging of nitric oxide signalling in thalamic neurons
  24. Hirth-Dietrich, Claudia, Cristina Alonso-Alija, Michael Härter, Michael G. Hahn, Yvonne Keim, Frank Wunder, Andreas Knorr, Johannes-Peter Stasch
    Antifibrotic effects of an sGC activator in rat models of liver fibrosis
  25. Kilic, Ana, Sevdalina Yurukova, Stepan Gambaryan, Thomas Renné, Ulrich Walter, Melanie Voss, Wolfgang Dostmann, Michaela Kuhn
    Increased tissue expression and activity of cGMP-dependent protein kinase I in ANP-receptor (GC-A) - deficient mice
  26. Kirsch, Mark, Norbert Weissmann, Barbara Kemp-Harper, Friedrich Grimminger, Harald H.H.W. Schmidt
    The phosphodiesterase 5 inhibitor, sildenafil, further augments the compensatory upregulation of NO-cGMP signalling in hypoxic pulmonary hypertension
  27. Klimenko, Oxana and Anna Matvienko
    The influence of peroxynitrite on some blood indices
  28. Kruuse, Christina, Tejvir S Khurana, Sergei D Rybalkin, Steffen Birk, Ulla Engel, Lars Edvinsson, Jes Olesen
    Phosphodiestrase 5 and effects of sildenafil on cerebral arteries from man and guinea pig
  29. Lakics, Viktor, Eric Karran, Frank G. Boess
    Analysis of phosphodiesterase expression using quantitative real time PCR
  30. Lange, Alexander, Florian Müllershausen, Andreas Friebe, Doris Koesling
    Desensitisation of NO/cGMP signalling in vascular and bronchial smooth muscle
  31. Langmesser, Sonja, Susanne Feil, Franz Hofmann, Robert Feil, Urs Albrecht
    Inactivation of cGKI in the CNS causes adaptation defects in the circadian clock in mice
  32. Lukowski, Robert, Pascal Weinmeister, Annette Vogl, Susanne Feil, Michael Gotthardt, Joachim Herz, Steffen Massberg, Franz Hofmann, Robert Feil
    Function of smooth muscle cGMP-dependent protein kinase type I in a mouse model of restenosis
  33. Lyamina, Nedezhda, V.N. Senchihin, Svetlana Lyamina
    The NO level in young patients with arterial hypertension and metabolic disorders
  34. Marro, Martin L. and Adrian J. Hobbs
    Characterisation of human α 1 and β 1 soluble guanylate cyclase promoter activity in human aortic smooth muscle cells
  35. Martin, Emil, Vasanthi Jayaraman, James Kincaid, Ferid Murad
    Resonance Raman and infrared spectroscopic studies of high-output forms of human soluble guanylyl cyclase
  36. Masuyama, Hiroyuki, Toshihiro Tsuruda, Johji Kato, Takuroh Imamura, Yujiro Asada, Johannes-Peter Stasch, Tanenao Eto
    Beneficial effect of s oluble guanylate cyclase stimulator BAY 41-2272 on c ardiovascular remodeling in angiotensin II-induced hypertensive rats
  37. Matt, Simone, Sabine Meurer, Steffen Gross, Werner Müller-Esterl
    Characterization of MRIP2 as a NO-sensitive guanylyl cyclase-associated protein
  38. Mergia, Evanthia, Andreas Friebe, Oliver Dangel, Michael Russwurm, Doris Koesling
    Conditional inactivation of the NO-sensitive guanylyl cyclase isoforms (α 1, β 1, α 2, β 1) in mice
  39. Meurer, Sabine, Steffen Gross, Sylke Pioch, Werner Müller-Esterl
    Tyrosine phosphorylation of NO-sensitive guanylyl cyclase
  40. Nedvetskaya, Tatiana Y., Pavel I. Nedvetsky, Sanchaita Sriwal Sonar, Helmut Müller, Albert Sickmann, Peter M. Schmidt, Petra Kronich, Johannes-Peter Stasch, Harald H.H.W. Schmidt
    Formation of quasi-covalent sGC a 1 / b 1 -heterodimers by ODQ-induced oxidation of the prosthetic heme moiety
  41. Nimmegers, Sofie, Patrick Sips, Emmanuel Buys, Peter Brouckaert, Johan Van de Voorde
    NO-mediated vascular smooth muscle relaxation in sGCα 1 knock-out mice
  42. Papapetropoulos, Andreas, Davina de C.M. Simoes, Georgia Xanthou, Charis Roussos, Christina Gratziou
    Reduction of soluble guanylyl cyclase expression in asthma
  43. Pioch, Sylke, Sabine Meurer, Steffen Gross, Werner Müller-Esterl
    Interaction of NO-sensitive guanylyl cyclase with Src-like kinases
  44. Postnikov, Alexander, Vasily Betin, Ferid Murad, Alexander Kots
    Regulation of soluble guanylate cyclase activity by direct interaction with heat shock protein Hsp90
  45. Pullamsetti, Soni, Ladislau Kiss, Hossein Ardeschir Ghofrani, Robert Voswinckel, Peter Haredza, Walter Klepetko, Clemens Aigner, Ludger Fink, Jai Prakash Muyal, Norbert Weissmann, Friedrich Grimminger, Werner Seeger, Ralph Theo Schermuly
    Increased levels and reduced catabolism of asymmetric and symmetric dimethylarginines in pulmonary hypertension
  46. Pyriochou, Anastasia, Chris Polytarchou, Evangelia Papadimitriou, Andreas Papapetropoulos
    Soluble guanylyl cyclase activation promotes angiogenesis
  47. Russwurm, Michael, Corina Wagner, Doris Koesling
    NO-sensitive guanylyl cyclase: binding and dissociation of nitric oxide
  48. Sausbier, Matthias, Hong Zhao, Rudolf Schubert, Ulrike Sausbier, U Abdullah, Andrea Huber, Susi Feil, Robert Feil, Franz Hofmann, Winfried Neuhuber, Hans-Dieter Allescher, Peter Ruth
    Enhanced vascular cGMP/cGK I signaling and hypotonia in cysteine-rich-protein 2-deficient mice
  49. Schmidt, Peter Manuel, Christiane Rothkegel, Frank Wunder, Friederike Stoll, Henning Schröder, Johannes‑Peter Stasch
    Residues stabilizing the heme moiety of the NO sensor soluble guanylate cyclase
  50. Schmidtko, Achim, Irmgard Tegeder, Ellen Niederberger, Franz Hofmann, Peter Ruth, Gerd Geisslinger
    The role of cGMP and PKG-I in spinal nociceptive processing
  51. Siednienko, Jakub, Małgorzata Ciuman, Hanna Witwicka, Wojciech A. Gorczyca
    Activation of NF-κB by nitric oxide/cGMP in human blood CD14 + cells
  52. Sips, Patrick, Emmanuel Buys, Robrecht Thoonen, Anje Cauwels, Elke Rogge 1, Maureen Van Den Hemel, Mieke Dewerchin, Peter Brouckaert
    Gender-specific hypertension in mice deficient in the alpha1 subunit of soluble guanylate cyclase
  53. Sonar, Sanchaita Sriwal, Thomas Eucker, Harald H.H.W. Schmidt The elusive b 2 subunit of sGC
  54. Tokuda, Harukuni, Fumio Enjo, Ayako Kumagai, Takao Konoshima, Midori Takasaki, Junko Takayasu, Hoyoku Nishino
    Tumor initiating activity of NO donor in two-stage mouse skin carcinogenesis and its role of cGMP
  55. Torres, Magdalena, Sandra Jurado, Elena López, Francisco M. Reimunde, Santiago Lamas, Fernando Rodríguez-Pascual
    NMDA treatment stabilizes the mRNA encoding for α 2 subunit of the NO-sensitive guanylyl cyclase by decreasing AUF1 protein level through a NO-cGMP-dependent mechanism
  56. Vanneste, Gwen, Ingeborg Dhaese, Patrice Sips, Emmanuel Buys, Peter Brouckaert, Romain A. Lefebvre
    Role of soluble guanylate cyclase in NO-induced effects of gastric fundus
  57. Wagner, Corina, Michael Russwurm, Doris Koesling
    NO-sensitive guanylyl cyclase: Identification and purification of the dimerization domain
  58. Wang, Yingrui, Stephanie Krämer, Tanja Loof, Sebastian Martini, Susanne Kron, Hiroshi Kawachi, Fuijo Shimizu, Hans-H. Neumayer, Harm Peters
    Enhancing cGMP in anti-thy1-induced, chronic-progressive glomerulosclerosis: sGC stimulation versus PDEs inhibition
  59. Weber, Silke, Susanne Feil, Sabine Brummer, Anna-Maria Knorn, Franz Hofmann, Robert Feil
    Knock-in mice expressing cGKIα or cGKIβ selectively in smooth muscle
  60. Wegener, Jörg W., Petra Smital, Jens Schlossmann, Franz Hofmann
    IRAG is involved in cGMP/cGK-mediated relaxation of contractions induced by calcium entry
  61. Weidenbach, Andreas, Johannes-Peter Stasch, Hossein Ardeschir Ghofrani, Norbert Weissmann, Friedrich Grimminger, Werner Seeger, Ralph Theo Schermuly
    Inhaled NO and the guanylate cyclase stimulator Bay 41-2272 in oleic acid induced acute lung injury in rabbits
  62. Weinmeister, Pascal, Robert Lukowski, Stefan Linder, Wolfgang Erl, Richard Brandl, Susanne Feil, Franz Hofmann, Robert Feil
    Regulation of vascular smooth muscle growth by cyclic nucleotides and cGMP-dependent protein kinase
  63. Wörner, René, Robert Feil, Franz Hofmann, Jörg W. Wegener
    Protein kinase A mediates 8-Br-cGMP-induced Ca 2+-desensitization in murine aorta
  64. Wunder, Frank, Adrian Tersteegen, Martin Hendrix
    Characterization of the novel PDE9 inhibitor BAY 73-6691 using a cGMP reporter cell line
  65. Zhou, Zongmin, Christina Gerassimou, Richard C. Venema, Charis Roussos , William C. Sessa, John Catravas, Andreas Papapetropoulos
    Interaction between hsp90 and soluble guanylyl cyclase: physiological significance and mapping of the domains mediating binding